Precise editing of human hematopoietic stem and progenitor cells (HSPC) is of interest both for the treatment of genetic disorders and for disease modeling. The advent of CRISPR/Cas has made such editing possible, however, current strategies have remained limited to 10-20% efficiency. Uses requiring purity thus need to include selection markers. This also limits donor selection to adeno-associated virus (AAV), as single stranded oligonucleotide donors (ssODNs) are not long enough to incorp...

David Knapp

Speaker

Genome-reduced cells have a lower number of genes that makes them especially interesting for systems and synthetic biology. Yet, genome reduction entails intense genomic modifications that often result in growth defects or other undesirable phenotypes. Using a suite of tools including adaptive evolution, metabolic modeling, and machine learning, we were able to diagnose and fix a constitutive oxidative stress response occurring in Escherichia coli DGF-298. This strain derives from E. coli ...

Sébastien Rodrigue

Speaker