Session 1: Metabolic Engineering and Bioprocessing
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The non-conventional yeast Yarrowia lipolytica is gaining interest in biotechnology as host for production of proteins, lipids and other biomolecules thanks to its capacity to achieve high biomasses, and to express high titers of proteins and lipids. Site-specific genome editing is however limited in this yeast. This was much improved by the recent development of a CRISPR-Cas9 genome editing protocol for Y. lipolytica based on a tRNA-sgRNA fusion. Nonetheless, in the latter protocol, Cas9 ...
Bioprocess development for biofuels and biochemicals typically requires several rounds of metabolic engineering to meet process targets including product yield, titer and productivity, all of which impact the process economics. Advances in computational modeling techniques have allowed the development of genome-scale models of metabolism in several organisms. Such models have been the basis of several algorithms that coup...
