Sessions in which David Knapp participates
Monday 12 June, 2023
Time Zone: (GMT-05:00) Eastern Time (US & Canada)
1:30 PM
1:30 PM -
2:00 PM |
30 minutes
Precise editing of human hematopoietic stem and progenitor cells (HSPC) is of interest both for the treatment of genetic disorders and for disease modeling. The advent of CRISPR/Cas has made such editing possible, however, current strategies have remained limited to 10-20% efficiency. Uses requiring purity thus need to include selection markers. This also limits donor selection to adeno-associated virus (AAV), as single stranded oligonucleotide donors (ssODNs) are not long enough to incorp...