The Next Scale of DNA Assembly, iPSC Culturing & Editing

Nicholas Gold¹,  Kevin Jacquet¹,  Flavia Araujo¹,  Ryo Niwa^2,3,  James Bagley¹,  Angela Quach¹, Benjamin Scott¹,  Knut Woltjen³,  Vincent Martin^1,4

1.  Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada

2.  Graduate School of Medicine, Kyoto University, Kyoto, Japan

3.  Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan

4.  Department of Biology, Concordia University, Montréal, QC, Canada

The Concordia Genome Foundry has a unique blend of expertise, spanning high-throughput DNA assembly, microbial engineering, and mammalian cell engineering and phenotyping. Combined with years of practical experience in laboratory automation, and a custom-built LIMS, the Genome Foundry is now well positioned to offer services to clients outside our university. Our poster provides an overview of our capabilities, along with several key case studies: 1) A 384-plasmid assembly, completed in under two weeks; 2) A 96-plasmid assembly that required maxi-prep quality DNA for iPSC transfection; 3) How our custom-built LIMS supports DNA assembly workflows; 4) Individual nucleofection of iPSC cells with 96 prime-editing plasmids, followed by direct comparisons of prime-editing efficiencies across all 96 plasmid designs. We also showcase capacities we’re building towards and include a video demonstration of our Mammalian Suite’s capabilities.